Part:BBa_K1988011:Design

his-tagged PPGK ATPi homologue w/ Anderson Promoter J23106

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 373
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 197
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimized for E. coli K-12 using IDT Codon Optimizer

Source

Genetic sequence from 'Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus Phosphovorus' by Kawakoshi, et. al.